Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: Global gene expression profiling and validation in esophageal squamous cell carcinoma (ESCC) and its association with clinical phenotypes

doi: 10.1158/1078-0432.CCR-10-2724

Figure Lengend Snippet: Summary of characteristics of patients in the 4 groups studied

Article Snippet: In addition to the directionality of the changes, the magnitude of the changes was also very similar: changes were 2-fold or greater for 10 of 13 (77%) up-regulated genes on both platforms, while 19 of 28 (68%) down-regulated genes showed fold changes of 0.50 or less on both platforms. table ft1 table-wrap mode="anchored" t5 caption a7 No. Gene Fold change by microarray type * , † 8K cDNA array (N=19) Affymetrix U133A/B array (N=53) Affymetrix U133A v2.0 array (N=17) Over-expressed genes 1 COL3A1 2.91 4.48 6.96 2 COL7A1 2.31 2.65 2.36 3 KRT14 2.31 2.50 2.60 4 FSCN1 2.22 3.06 2.96 5 SPARC 2.08 3.45 4.40 6 LAMC2 2.06 3.28 5.67 7 TAGLN2 2.06 1.54 1.30 8 FADD 2.04 2.30 5.44 9 CST1 2.04 3.47 7.40 10 HLA-B 2.03 1.43 1.56 11 CXCL10 2.03 1.88 6.69 12 CDC25B 2.01 2.22 3.49 13 COL1A2 2.01 6.49 11.82 Under-expressed genes 14 KRT4 0.16 0.17 0.13 15 TGM3 0.21 0.10 0.07 16 CSTA 0.26 0.53 0.53 17 CRCT1 0.27 0.11 0.06 18 SPRR1A 0.28 0.38 0.35 19 FOSL2 0.29 NA 0.64 20 UPK1A 0.29 0.32 0.17 21 EMP1 0.31 0.24 0.15 22 SPINK5 0.31 0.14 0.08 23 CSTB 0.34 0.28 0.39 24 C10orf116 0.34 0.27 NA 25 PRR4 0.35 0.42 0.92 26 SLURP1 0.36 0.13 0.06 27 KLK13 0.38 0.19 0.21 28 S100A9 0.38 0.45 0.35 29 CNN3 0.38 0.47 0.29 30 BTC 0.38 0.56 0.80 31 HEMGN 0.40 0.97 NA 32 PPL 0.41 0.24 0.17 33 EGR1 0.43 0.56 1.19 34 APC2 0.44 0.92 0.99 35 KLK11 0.45 0.33 0.24 36 HPGD 0.45 0.16 0.19 37 CD3EAP 0.47 1.33 1.61 38 DUSP5 0.47 0.27 0.21 39 EVPL 0.48 0.37 0.21 40 RARB 0.48 0.86 NA 41 CD48 0.50 0.91 0.78 Open in a separate window * NA = not available † Gene order based on 8K cDNA array values caption a8 Comparison of 41 dysregulated genes from previous 8K cDNA microarray with 2 Affymetrix microarrays A comparison of different RNA extraction methods applied to the Affymetrix platforms showed that, of 38 genes examined on both Affymetrix platforms, only one had a different expression pattern ( ).

Techniques: Microarray

Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: Global gene expression profiling and validation in esophageal squamous cell carcinoma (ESCC) and its association with clinical phenotypes

doi: 10.1158/1078-0432.CCR-10-2724

Figure Lengend Snippet: Comparison of 41 dysregulated genes from previous 8K cDNA microarray with 2 Affymetrix microarrays

Article Snippet: In addition to the directionality of the changes, the magnitude of the changes was also very similar: changes were 2-fold or greater for 10 of 13 (77%) up-regulated genes on both platforms, while 19 of 28 (68%) down-regulated genes showed fold changes of 0.50 or less on both platforms. table ft1 table-wrap mode="anchored" t5 caption a7 No. Gene Fold change by microarray type * , † 8K cDNA array (N=19) Affymetrix U133A/B array (N=53) Affymetrix U133A v2.0 array (N=17) Over-expressed genes 1 COL3A1 2.91 4.48 6.96 2 COL7A1 2.31 2.65 2.36 3 KRT14 2.31 2.50 2.60 4 FSCN1 2.22 3.06 2.96 5 SPARC 2.08 3.45 4.40 6 LAMC2 2.06 3.28 5.67 7 TAGLN2 2.06 1.54 1.30 8 FADD 2.04 2.30 5.44 9 CST1 2.04 3.47 7.40 10 HLA-B 2.03 1.43 1.56 11 CXCL10 2.03 1.88 6.69 12 CDC25B 2.01 2.22 3.49 13 COL1A2 2.01 6.49 11.82 Under-expressed genes 14 KRT4 0.16 0.17 0.13 15 TGM3 0.21 0.10 0.07 16 CSTA 0.26 0.53 0.53 17 CRCT1 0.27 0.11 0.06 18 SPRR1A 0.28 0.38 0.35 19 FOSL2 0.29 NA 0.64 20 UPK1A 0.29 0.32 0.17 21 EMP1 0.31 0.24 0.15 22 SPINK5 0.31 0.14 0.08 23 CSTB 0.34 0.28 0.39 24 C10orf116 0.34 0.27 NA 25 PRR4 0.35 0.42 0.92 26 SLURP1 0.36 0.13 0.06 27 KLK13 0.38 0.19 0.21 28 S100A9 0.38 0.45 0.35 29 CNN3 0.38 0.47 0.29 30 BTC 0.38 0.56 0.80 31 HEMGN 0.40 0.97 NA 32 PPL 0.41 0.24 0.17 33 EGR1 0.43 0.56 1.19 34 APC2 0.44 0.92 0.99 35 KLK11 0.45 0.33 0.24 36 HPGD 0.45 0.16 0.19 37 CD3EAP 0.47 1.33 1.61 38 DUSP5 0.47 0.27 0.21 39 EVPL 0.48 0.37 0.21 40 RARB 0.48 0.86 NA 41 CD48 0.50 0.91 0.78 Open in a separate window * NA = not available † Gene order based on 8K cDNA array values caption a8 Comparison of 41 dysregulated genes from previous 8K cDNA microarray with 2 Affymetrix microarrays A comparison of different RNA extraction methods applied to the Affymetrix platforms showed that, of 38 genes examined on both Affymetrix platforms, only one had a different expression pattern ( ).

Techniques: Microarray

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: Mir-434-5p mediates skin whitening and lightening

doi:

Figure Lengend Snippet: Human genome microarray analyses (Affymetrix human GeneChip U133A&B, CA) of altered gene expression patterns in the human primary skin cell cultures with or without miR-Tyr transfection. This procedure was performed to demonstrate the higher target-specificity and lower off-target effect of the miR-Tyr compared to those of native microRNAs, such as mir-434-5p.

Article Snippet: Sets of four oligonucleotide microarrays (GeneChip U133A&B arrays, Affymetrix, Santa Clara, CA) containing total 32,668 genes were used for hybridization.

Techniques: Microarray, Expressing, Transfection